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What Is EDC HCl (EDC Hydrochloride)?
EDC HCl (EDC Hydrochloride) (CAS 25952-53-8) is a carbodiimide coupling reagent with the molecular formula C8H17N3 HCl and molecular weight 191.70 g/mol. [001]
Physical and Chemical Properties
| Property | Value | Source |
|---|---|---|
| CAS Number | 25952-53-8 | |
| Molecular Formula | C8H17N3 HCl | |
| Molecular Weight | 191.70 g/mol |
Key Applications
- Amide/Peptide Bond Formation: EDC activates carboxylic acids via O-acylisourea intermediate for amide bond formation. The EDC urea byproduct is water-soluble -- simpler workup than DCC. Typical: 1.1-1.5 eq EDC, 1.1 eq HOBt to suppress racemization, DMF or CH2Cl2, 0C to rt. [004]
- Bioconjugation & Protein Crosslinking: EDC is the standard reagent for conjugating carboxyl-containing biomolecules to amine surfaces/particles. EDC/NHS two-step protocol produces stable NHS esters for ELISA, SPR biosensor, and lateral flow assay development. [004]
- Esterification Under Mild Conditions: EDC-mediated esterification proceeds under neutral conditions -- compatible with acid-sensitive substrates. DMAP (5-10 mol%) is added as nucleophilic catalyst. [005]
- DNA Microarray Fabrication: EDC activates 5'-phosphate of oligonucleotides for coupling to amine-modified surfaces. Water-soluble urea byproduct simplifies purification. [005]
- Amide/Peptide Bond Formation: EDC activates carboxylic acids via O-acylisourea intermediate for amide bond formation. The EDC urea byproduct is water-soluble -- simpler workup than DCC. Typical: 1.1-1.5 eq EDC, 1.1 eq HOBt to suppress racemization, DMF or CH2Cl2, 0C to rt. [004]
- Bioconjugation & Protein Crosslinking: EDC is the standard reagent for conjugating carboxyl-containing biomolecules to amine surfaces/particles. EDC/NHS two-step protocol produces stable NHS esters for ELISA, SPR biosensor, and lateral flow assay development. [004]
- Esterification Under Mild Conditions: EDC-mediated esterification proceeds under neutral conditions -- compatible with acid-sensitive substrates. DMAP (5-10 mol%) is added as nucleophilic catalyst. [005]
- DNA Microarray Fabrication: EDC activates 5'-phosphate of oligonucleotides for coupling to amine-modified surfaces. Water-soluble urea byproduct simplifies purification. [005]
Additional Applications
Key Applications
Representative Protocol
Application: Amide/Peptide Bond Formation
Typical Scale: Laboratory scale (1-100 mmol). For industrial scale, consult process chemistry references.
General Procedure: For amide/peptide bond formation, follow established protocols from the chemical literature and supplier technical documentation. Key parameters: use anhydrous solvents and reagents where moisture sensitivity is indicated; monitor reaction progress by TLC, HPLC, or GC-MS; standard aqueous workup (extraction with appropriate organic solvent, washing with brine, drying over Na2SO4 or MgSO4); purify by column chromatography, recrystallization, or distillation as appropriate for the product. For specific substrate combinations and optimized conditions, consult the referenced SOURCE materials in this article.
Quality Control: Confirm product identity by 1H/13C NMR, HRMS or LC-MS, and compare to literature data. For chiral products, determine enantiomeric excess by chiral HPLC or NMR with chiral shift reagents.
Safety Note: Conduct all operations in a properly functioning fume hood with appropriate PPE. Quench reactive intermediates/excess reagents according to established protocols before workup. Dispose of chemical waste in accordance with institutional and local regulations.
Troubleshooting:
- Low conversion: Check reagent purity (especially moisture-sensitive reagents). Ensure anhydrous conditions. Increase catalyst loading or reaction temperature. Verify substrate quality by NMR.
- Side product formation: Lower reaction temperature. Use more selective reagents or catalysts. Consider protecting group strategy for sensitive functional groups.
- Product decomposition during workup: Neutralize acids/bases before extraction. Use mild drying agents. Avoid prolonged exposure to silica gel if the product is acid-sensitive.
Handling and Storage
- GHS Hazard: H317: May cause an allergic skin reaction, H319: Causes serious eye irritation, H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled
- Signal Word: Danger
- Pictograms: GHS05, GHS06, GHS07
- Storage: Store at 2-8 °C or as recommended. Keep container tightly closed in a cool, dry, well-ventilated area. Protect from moisture, heat, and direct sunlight. For compounds used in pharmaceutical or GMP applications, store in a dedicated area with controlled access and environmental monitoring. Always consult the supplier's SDS for lot-specific requirements.
- PPE: Nitrile gloves, safety goggles with side shields, lab coat, and closed-toe shoes. Work in a well-ventilated fume hood. Use respiratory protection (NIOSH-approved N95 or better) if handling large quantities or if the SDS indicates inhalation hazard. [002]
Always consult the Safety Data Sheet (SDS) from your supplier before handling. SDS documents are lot-specific — different purity grades and formulations may have different hazard classifications. For bulk handling, consult your institution's chemical hygiene plan and perform a job hazard analysis (JHA) before beginning work. Emergency procedures: in case of skin contact, wash immediately with soap and water for at least 15 minutes. For eye contact, rinse cautiously with water for several minutes and remove contact lenses if present. If inhaled, move to fresh air. Seek medical attention if symptoms persist.
Regulatory & Compliance
Chemical Inventories: EDC HCl (EDC Hydrochloride) (CAS 25952-53-8) is listed on major chemical inventories including the TSCA (Toxic Substances Control Act) inventory in the United States, EINECS (European Inventory of Existing Commercial Chemical Substances) in the EU, and similar inventories in Japan (ENCS), China (IECSC), Korea (KECL), and Australia (AICS). The specific regulatory status of CAS 25952-53-8 should be verified with the appropriate national authority before importing into any jurisdiction, as inventory listing status can change with regulatory updates and new chemical assessments.
GHS Classification: Refer to the Handling and Storage section above for the Globally Harmonized System hazard classification. The GHS classification is harmonized across most jurisdictions, but specific national implementations (EU CLP Regulation, US OSHA HazCom 2012, China GB 30000 series) may have additional requirements.
Transport Information: Verify the proper shipping name, UN number, hazard class, and packing group with your supplier before shipping. Transport regulations (IATA/ICAO for air, IMDG for sea, ADR/RID for road/rail in Europe, 49 CFR for US ground) may impose quantity limits, packaging requirements, and documentation obligations. Some compounds may be classified as dangerous goods requiring specialized handling and certified packaging.
Export Control: EDC HCl (EDC Hydrochloride) is a standard research and industrial chemical. However, always verify that no specific export control regulations apply to your destination country. Certain chemical categories (precursors, dual-use chemicals) may require export licenses under the Chemical Weapons Convention (CWC), Australia Group guidelines, or national export control laws. Contact your local trade compliance office for the most current regulatory guidance.
[004] [002]
Frequently Asked Questions
What is the CAS number of EDC HCl (EDC Hydrochloride)?
The CAS Registry Number is 25952-53-8. Always specify the CAS number when ordering to ensure correct compound identification regardless of naming conventions. [001]
What is the molecular weight of EDC HCl (EDC Hydrochloride)?
The molecular weight is 191.70 g/mol based on the molecular formula C8H17N3 HCl. Use this value to calculate molar equivalents, determine reaction stoichiometry, and prepare solutions of known concentration. [001]
What is EDC HCl (EDC Hydrochloride) used for?
EDC HCl (EDC Hydrochloride) is used for: Amide/Peptide Bond Formation (EDC activates carboxylic acids via O-acylisourea intermediate for amide bond for...); Bioconjugation & Protein Crosslinking (EDC is the standard reagent for conjugating carboxyl-containing biomolecules to ...); Esterification Under Mild Conditions (EDC-mediated esterification proceeds under neutral conditions -- compatible with...). For detailed protocols, see the Key Applications section above. [002]
How should EDC HCl (EDC Hydrochloride) be stored?
Store at 2-8 °C or as recommended. Keep container tightly closed in a cool, dry, well-ventilated area. Protect from moisture, heat, and direct sunlight. For compounds used in pharmaceutical or GMP applications, store in a dedicated area with controlled access and environmental monitoring. Always consult the supplier's SDS for lot-specific requirements.. Always consult the Safety Data Sheet (SDS) from your specific supplier for the most accurate storage recommendations. [002]
Where can I buy EDC HCl (EDC Hydrochloride)?
EDC HCl (EDC Hydrochloride) (CAS 25952-53-8) is available from CoreyChem. For bulk pricing, custom packaging, and a certificate of analysis, submit an inquiry to our sales team with your CAS number, required quantity, and desired purity. [003]
Where to Buy
EDC HCl (EDC Hydrochloride) is available in the CoreyChem catalog.
[View EDC HCl (EDC Hydrochloride) (CAS 25952-53-8) on CoreyChem →](https://careerchemical.com/product/25952-53-8) [003]
To request a quote, check bulk availability, or inquire about custom packaging for EDC HCl (EDC Hydrochloride), contact our sales team at [email protected] or submit an inquiry through our website. We respond to all inquiries within one business day with pricing and availability information tailored to your specific requirements.
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References & Sources
https://www.tcichemicals.com/US/en/search/?text=25952-53-8
https://pubchem.ncbi.nlm.nih.gov/compound/25952-53-8
https://pubchem.ncbi.nlm.nih.gov/compound/25952-53-8#section=Use-and-Manufacturing
